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c-terminal

Question tag: c-terminal 13 Questions

0 Votes 1 Ans

How can the C-terminal fusion vectors be used to label the C-terminus of the target protein?

c-terminal c-terminus Fusion Label protein target Used vectors

0 Votes 1 Ans

When do I need N-terminal acetylation or C-terminal amidation?

acetylation amidation c-terminal n-terminal

0 Votes 1 Ans

Which residues or sequence should be inserted at the cleavage site to improve controllable cleavage in C-terminal pTYB vectors (pTYB1,2,3 and 4)?

c-terminal cleavage controllable inserted ptyb ptyb123 residues sequence site vectors

0 Votes 1 Ans

How to design PCR Primers for cloning into C-terminal fusion vectors pTXB, pTYB (pTYB 1,2,3 and 4), or pCYB vectors?

c-terminal cloning design Fusion PCR Primers ptxb ptyb vectors

0 Votes 1 Ans

How should a cloning strategy for C-terminal fusions (pTXB1,3 and pTYB1,2,3,4) be designed if the gene of interest contains both NdeI and NcoI sites?

c-terminal cloning designed fusions gene ndei ptxb13 ptyb1234 strategy

0 Votes 1 Ans

Do my peptides need N-terminal acetylation and C-terminal amidation?

acetylation amidation c-terminal n-terminal peptides

0 Votes 1 Ans

How should a cloning strategy for C-terminal fusions (pTYB1,2,3,4 and pTXB1,3) be designed if the gene of interest contains both NdeI and NcoI sites?

c-terminal cloning designed fusions gene ndei ptxb13 ptyb1234 strategy

0 Votes 1 Ans

Are there known ways to accelerate the hydrolysis of DTT from the C-terminal of the target protein in a controlled manner?

accelerate c-terminal controlled DTT hydrolysis known manner protein target

0 Votes 1 Ans

In the case of the C-terminal fusion vectors (pTYB1,2,3 and 4), which residues at the C-terminal of the target protein may inhibit cleavage or cause in vivo cleavage?

c-terminal Case Fusion inhibit protein ptyb123 residues target vectors

0 Votes 1 Ans

In the case of C-terminal fusion vectors, if the EcoRI site is used as the 3´ cloning site, which vector should be chosen?

c-terminal Case cloning ecori Fusion site Used vector vectors

0 Votes 1 Ans

How is the C-terminal end of Micro-Scale Peptides defined?

c-terminal defined micro-scale peptides

0 Votes 1 Ans

How can a C-terminal 6xHis-tagged QIAgene be expressed?

c-terminal expressed

0 Votes 1 Ans

How is the C-terminal end of BioTides™ defined?

c-terminal defined