Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Are Methyl-Profiler DNA methylation PCR Arrays or Assays applicable to FFPE samples?

April 26, 2017applicable arrays assays dna ffpe methyl-profiler Methylation PCR samples

0

Posted

Are Methyl-Profiler DNA methylation PCR Arrays or Assays applicable to FFPE samples?

1 Answer

0

Posted

NO. Under current protocol, FFPE samples are not suitable for Methyl-Profiler qPCR Arrays and Assays. It is because genomic DNA from FFPE samples is single stranded due to the heat process involved in genomic DNA isolation process, thus is indigestible with either Methylation-Sensitive or Methylation-dependent enzyme. Please double check the User Manual of your chosen genomic DNA isolation kit. If there is 95C heating step involved, please do not use the kit to isolate genomic DNA for Methyl-Profiler qPCR Arrays or Assays. Instead, use the recommended genomic DNA isolation Kit in the User Manual of Methyl-Profiler qPCR Arrays or Assays.