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Are the gel spots suitable for mass spectrometry based protein identification once excised from the polyacrylamide gel matrix?

April 26, 2017based excised gel Identification mass polyacrylamide protein spectrometry spots suitable

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Are the gel spots suitable for mass spectrometry based protein identification once excised from the polyacrylamide gel matrix?

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Yes, the spots are cut from the gel robotically and deposited into a 1% acetic acid solution for storage. They are subsequently digested with trypsin and sent for mass spectrometry analysis by a MALDI-TOF or LTQ, upon request.