Are there differences between conventional HPLC data treatment and radio-HPLC data treatment?
Because radioactive decay is a statistical process, we try to make as long a measurement as possible to obtain a good average. Therefore, we use relatively large cells — 1000 ul is perhaps average — vs. 5-10 ul cells for other HPLC detectors; we might even use larger cells if we could but we are limited by the possibility of more than one peak being present in the cell at any one time. With such large cells, we make suitable corrections for flow, and for dilution with scintillator solution if used, that are not normally made with other detectors. Normally, all such corrections are built into the radio-HPLC software that accompanies most instruments; they take place automatically, and are transparent to the user. As a further consequence of the use of large cells for radioactivity detection and small ones for mass, when both are used in the same system, there are substantial time displacements of the peaks resulting from the fact that the two detectors are in series and one measuremen
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