Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can a different promoter other than SP6 be used for the wheat germ cell-free expression system?

April 26, 2017cell-free different expression Germ promoter sp6 system Used wheat

0

10 Posted

Can a different promoter other than SP6 be used for the wheat germ cell-free expression system?

1 Answer

0

10 Posted

In principle, we recommend the use of SP6 promoter inserted in the pEU vector. T7 promoter can be used, but the yield for the same mole of transcript would be lower. This is attributed to the difference in the 5′ transcript site sequence between SP6 and T7 promoters.