Yes, if you have two floppy drives and the new Disk2FDI program which will read Amiga Disks and write it to FDI format which is compatible with WinUAE. For the PC to read Amiga disks directly, extra hardware will need to be purchased which can read (or write) Amiga disks easily. The ISA Catweasel floppy controller can be used on the PC to read (or write) Amiga disks. Further information is available from the web site at Individual Computers. Finally, if these options are not feasible, why not buy a real Amiga second hand for those occasions when you want to transfer files and you'll have access to a real KS and WB for use on your main machine.