Can bands be seen on a UV transilluminator without the use of emission filters?
Bands stained with SYBR® Safe DNA gel stain are visible to the eye on a 300 nm transilluminator. However, optimum detection is obtained by photographing the gel using a UV-compatible emission filter with your CCD or film camera. UV bulbs may also emit some IR; if your camera lens is not specially coated to block IR, an IR-blocking filter is needed to prevent the appearance of faint images of the UV bulbs behind your gel. For optimum detection and complete safety, use the Safe Imager™ blue-light transilluminator.
