Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I compare Beta values calculated in BeadStudio to percent methylation calculated using other technologies?

April 26, 2017beadstudio beta calculated compare Methylation percent technologies values

0

Posted

Can I compare Beta values calculated in BeadStudio to percent methylation calculated using other technologies?

1 Answer

0

Posted

The Beta value is unique to the Illumina platform and can range from 0 to 1, with 0 indicating no methylation and 1 indicating complete methylation at a given locus. Importantly, the Beta value reports on the intensity ratio between methylated- and nonmethylated-specific assays for each CpG site. Since assay performance can vary between loci, this ratio does not consistently report percent methylation.