Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I distinguish between the binding of an inhibitor vs. the binding of a substrate to an enzyme?

April 26, 2017Binding distinguish enzyme inhibitor Substrate vs

0

Posted

Can I distinguish between the binding of an inhibitor vs. the binding of a substrate to an enzyme?

1 Answer

0

Posted

You will be able to distinguish it from the signal amplitudes. As an example, you can first saturate a kinase with a non-hydrolysable ATP analog and add different concentrations of a kinase inhibitor. The displacement of the ATP analog will cause a change in thermophoresis.