Can I generate antisera against gel strips containing my protein?
• Yes, immunizing with gel bands works well for generating polyclonal antisera against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands in particular, it is important to maintain a higher concentration of the protein as outlined above.
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- Can I generate antisera against gel strips containing my protein?
