Can I load the IMAPlate with several rounds of pipetting?
A5: Yes, you can pipette IMAPlate several times. But the total volume per well should not excess 6 µl. In order to not make bubbles, the tip end should be in the solution, which is already inside the reaction chamber, before dispensing the solution. Q6. How can I measure DNA/RNA and protein absorbance by using IMAPlate 5RC96 as a micro-UV-cuvette array? What about the CV%? A6: You should always use the two-wavelength measurement method in order to get true absorbance. It is recommended to use the same liquid loading method for standards and samples in order to get accurate results. The CV% is usually around 5% for a sample in the plate by using capillary force loading method. Q7. Can I use the white IMAPlate 5RC96 for fluorescence measurement? A7: Yes, but the background signal will be high. Use the black IMAPlate for fluorescence measurement. Q8. Can I reduce assay time by using the IMAPlate 5RC96 to replace 96-well plate? A8: Of course, when the reaction is heterogeneous and uses the
