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Can I repeatedly thaw and refreeze microsomes and S9 if I only need to remove a small aliquot of these products from a vial?

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Can I repeatedly thaw and refreeze microsomes and S9 if I only need to remove a small aliquot of these products from a vial?

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Yes, as long as you carefully thaw these products on ice, remove what you need, and then quickly refreeze the vial to -70° C. As an alternative to repeated thawing and freezing, you can thaw the vial once and distribute the contents into single-use vials for future use. That way, you only have to thaw the microsomes/S9 one more time.

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