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Can I use a total RNA input of less than 20 ng?

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Can I use a total RNA input of less than 20 ng?

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The Ovation™ RNA Amplification System V2 has been validated for total RNA input amounts of 5 to 100 ng, however for whole blood amplifications we strongly recommend staying within the 20-50 ng range. Using lower input amounts will result in lower performance.

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