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Can I use agarose gels or must I use acrylamide gels?

April 26, 2017acrylamide agarose gels

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Can I use agarose gels or must I use acrylamide gels?

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EMSAs may be performed with either polyacrylamide or agarose gels depending upon the resolution requirements of the study system. Traditionally, 4-6% non-denaturing polyacrylamide gels are used. If agarose is used, a capillary transfer may be best. Either capillary or electrical transfers can be performed with polyacrylamide gels.