CAN I USE BOTH SINGLE-COLOR AND DUAL-COLOR HYBRIDIZATION APPROACHES? HOW DO I LABEL NUCLEIC ACID TARGETS FOR HYBRIDIZATION WITH CUSTOMARRAY CHIPS?
Yes, both approaches work for CustomArray chips. For the single-color approach we recommend to label nucleic acid targets by biotin incorporation, to hybridize arrays with the biotinylated targets, and then to perform post-hybridization labeling using streptavidin-conjugated fluorescent dye (e.g. Fluorolink Cy5-labeled streptavidin from Amersham, Catalog # PA45001). For the dual-color approach, two target nucleic acid samples may be directly labeled with two different fluorescent dyes (e.g. Cy3 and Cy5) during amplification reactions using commercially available kits (e.g. Ambion kits for cRNA and cDNA labeling). For gene expression applications with eukaryotic material we recommend to use the Ambion MessageAmp™ II aRNA Amplification Kit (Catalog #1751).
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