Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I use the Zymo kit for DNA purification after the immunoprecipitation?

April 26, 2017dna immunoprecipitation kit purification

0

Posted

Can I use the Zymo kit for DNA purification after the immunoprecipitation?

1 Answer

0

Posted

As the DNA purification step is critical and the loss of precious DNA might occur at this step, we recommend our optimized protocol from the MeDIP kit. Recently we have compared different DNA purification methods of immunoprecipitated DNA. We can conclude that Phenol/chloroform & ETOH precipitation dramatically improved our yield (93%) of methylated product (qPCR data) compared to the use of Zymo (43%) and Qiagen (52%) columns.