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Can Ni-NTA resins be used to purify protein with an internal His-tag?

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Can Ni-NTA resins be used to purify protein with an internal His-tag?

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Yes, Ni-NTA Agarose and Superflow will bind a 6xHis-tag whether it is located internally or at the C- or N-teminal end of the protein. Note that the His-tag must be exposed for binding at the sur…

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