Can proteins be transferred to a membrane from a gel after they have been stained by BlueFast™?
No for most proteins. Once proteins are stained by BlueFast™, they can not be transferred. It is misleading to see blue bands on a membrane if one attempts to transfer BlueFast™ stained proteins from gel. The transferred blue bands are coomassie blue dyes. Since protein detection by BlueFast™ is so rapid, we recommend running two identical gels simultaneously. Leave one gel in the electrophoresis apparatus, stain the second gel with BlueFast™ and obtain the results within 10 minutes. Based on the results of the stained gel, the user then can decide whether to transfer the unstained gel to a membrane. Q: Can I remove the light-blue background produced by BlueFast™ completely? A: BlueFast™ leaves a light-blue background, which does not affect the sensitivity of detection on the gel. To completely remove the light-blue background, one can repeat the post staining wash step with heating several times, leave the gel in 500 ml of water and change the water hourly, or simply leave the gel in