Can the IMPACT System be used to purify peptides?
The IMPACT System provides an attractive alternative method for peptide synthesis and purification. In each case, expression, stability and inducible cleavage of the fusion have to be optimized. It is also possible to use this system to study peptide interactions with other proteins or ligands. We believe that it is possible to purify peptides using the IMPACT system but you need to consider two major factors. The first one is that certain residues or sequences may not exhibit efficient cleavage activity; the cleavage efficiency of different amino acid residues flanking the cleavage site varies depending on the target and intein sequence. The second factor is that the yield for peptides could be low due to their small mass. Furthermore, the cleavage efficiency of each intein-target protein fusion may vary. For peptide expression using the IMPACT system please refer to the following reference – Evans, T.C. Jr., Benner, J., and Xu, M.-Q. 1999. The cyclization and polymerization of bacter
