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No, the design of TripleFlask does not allow for mechanical scraping. However, cells are easily harvested using protein digesting enzymes, such as trypsin, collagenase, and pronase or by using non-enzymatic dissociation solutions. Tech Note Vol. 1 No. 2 describes culturing techniques for Nunclon Δ TripleFlask.
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Can the Nunc Cell Scrapers be used with the Nunclon Δ TripleFlask?
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