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Can the sensitivity be increased by altering the Detection Antibody Cocktail or Streptavidin-HRP concentration?

April 26, 2017altering Antibody cocktail concentration detection increased

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Can the sensitivity be increased by altering the Detection Antibody Cocktail or Streptavidin-HRP concentration?

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Use the recommended concentrations of Detection Antibody Cocktail and Streptavidin-HRP. Using a higher concentration of either may result in high background. Longer exposures to X-ray film (5 to 10 minutes) should help to increase sensitivity.