Can the Strep-tag® be cleaved off?
Yes. However, this is only recommended if absolutely no modification on the recombinant protein is tolerated. Due to its small size and chemically inert nature, Strep-tag II does generally not interfere with the folding or bioactivity of the recombinant protein. • What are the optimal cleavage conditions to remove the Strep-tag®? The cleavage efficiency depends on the accessibility of the cleavage site which is influenced by the proximal 3D structure of the recombinant protein. Therefore, the necessary amount of protease may vary greatly and can not be predicted. • Do you have a kit for first users? Yes, we have several Starter-kits [Cat.-no.: 2-1101-000, 2-1102-001, 2-1103-001, 2-1104-000, 2-1115-000, 2-1116-000]. The kits come with different amounts of columns or cartridges and include all necessary buffers for expression, purification (binding, washing, elution, regeneration) and detection for the first eight applications in a 100 ml E. coli culture scale. • Which protein serves in
