Can the yield be increased by adding additional GEMs during the inoculation phase?
Yes, more GEMs may be used during the inoculation phase. The more surface area available for growth will result in a higher final yield. The limiting factor will be the medium. The more GEM and cells used in the inoculation phase will lead to more cells proliferating on the surface of the GEM and faster consumption of the medium components essential for growth. The optimum cell to GEM ratio should be identified for each cell type cultured.