Do any substances interfere with the binding of LPS to Detoxi-Gel Support?

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Do any substances interfere with the binding of LPS to Detoxi-Gel Support?

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Yes. Other detergents and high levels of chaotropes (urea and guanidine) can reduce the affinity of the Polymixin B for the LPS. Proteins such as BSA can bind tightly to endotoxin, reducing its ability to interact with and bind to the Detoxi-Gel Support. This reduction in binding capacity can sometimes be overcome by increasing the volume of Detoxi-Gel Support to LPS. However, some proteins bind tightly to endotoxin without inhibiting its binding to the support. In this case, the protein will remain bound to the gel with the endotoxin, possibly causing a loss of the protein of interest.

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