Do CD34 or CD133 MicroBeads interfere with proliferation and differentiation of hematopoietic cells in culture?

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Do CD34 or CD133 MicroBeads interfere with proliferation and differentiation of hematopoietic cells in culture?

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No, they do not. Cells can be cultured straight after separation with MACSĀ® Technology. Proliferation and differentiation of hematopoietic cells in different culture systems remain unaffected. However, prolonged storage of cells in EDTA-containing buffer may have a slightly negative effect on cell proliferation. EDTA can be replaced by other supplements, such as 0.6% ACD-A or citrate phosphate dextrose (CPD).

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