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Do you recommend purification of the cDNA prior to qPCR analysis?

analysis cDNA prior purification qPCR recommend
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Do you recommend purification of the cDNA prior to qPCR analysis?

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Yes. Although this is not typically necessary, it is important to be able to quantitate the amplified cDNA. This allows assessment of the amplification success based on the yields obtained, it also allows mass normalization of the cDNA into qPCR.

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