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Does a touchdown PCR protocol improve results?

PCR Protocol results Touchdown
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Does a touchdown PCR protocol improve results?

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No. Relative peak areas of the probe amplification products change depending on the PCR protocol used. Even though each fragment is amplified with the same PCR primers, some fragments have stronger reduced amplification efficiencies at annealing temperatures in excess of 64°C. The annealing temperature and the speed of temperature changes are often not perfectly uniformly distributed across the different parts of the thermocycler. The reproducibility of results may therefore be lower when the first PCR cycles have a low efficiency.

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