Does anyone have any references for Michels Fixative or Fluid?
We use it for an immunofluorescence holding medium, but I don’t have a reference on it for the manual … (and I would like to read about it just for my own knowledge). Answer. Here’s my procedure sheet for Michel’s transport medium. MICHEL’S TRANSPORT MEDIUM Michel’s transport medium (pronounced mee-SHELL) is used to transport specimens (such as renal biopsies and lymph nodes) for immunofluorescence studies. It is not a fixative, and is not suitable for any other use (particularly, it is not suitable for transporting living cells for flow cytometry). It should be stored refrigerated (not frozen) until use. Specimens may be kept in it at room temperature until they can be delivered to the reference laboratory. Zeus Medium, a commercial product, is probably similar. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled water. Cool. Adjust pH to 7.0 with 1 M potassium hydroxide (about 35 mL)
We use it for an immunofluorescence holding medium, but I don’t have a reference on it for the manual … (and I would like to read about it just for my own knowledge). Answer. Here’s my procedure sheet for Michel’s transport medium. MICHEL’S TRANSPORT MEDIUM Michel’s transport medium (pronounced mee-SHELL) is used to transport specimens (such as renal biopsies and lymph nodes) for immunofluorescence studies. It is not a fixative, and is not suitable for any other use (particularly, it is not suitable for transporting living cells for flow cytometry). It should be stored refrigerated (not frozen) until use. Specimens may be kept in it at room temperature until they can be delivered to the reference laboratory. Zeus Medium, a commercial product, is probably similar. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled water. Cool. Adjust pH to 7.0 with 1 M potassium hydroxide (about 35 mL)
