Does Biosearch measure A260/A280 ratios of the oligos?
No, we do not measure A260/A280 ratios at Biosearch. A260/A280 ratios are used for measuring protein to DNA concentration following applications in which protein contamination may be an issue, such as extractions from cells. It is not necessary for this ratio to be applied to synthetic DNA analysis as protein contamination is not a concern. See reference: Validity of nucleic acid purities monitored by 260 nm/280 nm absorbance ratio. Biotechniques. 18(1): p 62-63, 1995.