Does my oligonucleotide have a phosphate on the 5’ or 3’ end?
All of our standard custom oligonucleotides are synthesized with a hydroxyl group on both the 3’ and the 5’ ends. However, if requested, we can synthesize your oligo with a 5’ and/or 3’ phosphate. Dual-labeled fluorescent probes will not be extended as long as there is a quencher at the 3’ end. As for probes with internal quenchers, a 3’ phosphate will be added to eliminate undesired extension.
