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Q:

Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?

Answers

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Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production. The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist. more
ncbi.nlm.nih.gov
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