Does the vector pMM1522 contain a transcription terminator downstream of the MCS? Or do I need to insert a transcription terminator downstream of my gene of interest?

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Does the vector pMM1522 contain a transcription terminator downstream of the MCS? Or do I need to insert a transcription terminator downstream of my gene of interest?

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A.: No, pMM1522 and all other vectors of this series do not contain a terminator sequence. But it is not necessary to insert a terminator sequence. This was found in experiments comparing vectors with and without terminator. Here the terminator showed no effect on the amount of produced protein.

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