FBM loading media produce a light yellow band on my gels. How can I get a darker dye marker?
You have a number of options, and most of them will perform quite well. For many types of DNA samples, any dye will work and any loading media available to the researcher – from any source – usually will perform at least adequately. FBM uses orange G in the loading media. We utilize orange G because it will not obscure the visualization and photography of DNA bands as will bromphenol blue (BPB) and some other dyes. In order to optimally match the loading media to our 1X low-conductive solutions, we have set the concentration of the orange G rather low. We do this because all of the migrating dyes for electrophoresis are anions, usually supplied as a sodium salt. In order to provide maximal resolution, to minimize the effects of salt on DNA migration speed, and to provide maximal flexibility for users whose samples might have a bit too much salt (such as when analyzing unpurified PCR products), we minimized the salt in the loading medium and provide a sample of the FBM loading medium ma
