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fixed, decalcified, paraffin embedded bone sections?

bone embedded fixed paraffin
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fixed, decalcified, paraffin embedded bone sections?

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Answer 1. Have a go, I used to stain for acid phosphatase in 1-10 æm sections of demineralized, glutaraldehyde/osmium-fixed epoxy-embedded specimens with no bother. The method was nothing special, just a standard napthol AS-BI phosphate/diazotised pararosaniline technique.

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