For methylation microarray purpose, which protocol for DNA elution and purification is recommended?
DNA samples immunoprecipitated using MeDIP or MagMeDIP kit can be used successfully for microarray analysis. An average recovery after MeDIP (starting material is 1 µg) is about 20-50 ng of single-stranded DNA. As microarray study requires 1-2 µg of DNA, immunoprecipitated DNA should be amplified. If you work with MagMeDIP (cat # mc-magme A10 or A32), DNA isolated with DIB buffer can be used directly for qPCR but for other applications it is better to not use DIB buffer, but to perform additional purification: elution with our DNA purification module (cat # mc-magme-002) with following phenol-chloroform extraction or columns purification. Note anyway that columns purification results in significant DNA lost (about 30%).
Related Questions
- Which aspects of the recommended protocol are most critical to achieve optimal results when extracting DNA using the PrepFiler™ kit?
- Do you have a recommended protocol for front-end sample processing for producing DNA fragments enriched for methylation?
- What is the amount of DNA recommended for a microarray experiment?
