For my DNA concentration determination (A260) the BioPhotometer shows a negative value at 230 nm and as a result no ratio A260/A230. What causes the negative A230 value?
Negative A230 values are usually caused by interference components in inadequately concentrated DNA solutions. The negative value should be rectified when you use a lesser sample dilution for the next measurement. Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results.
Related Questions
- For my DNA concentration determination (A260) the BioPhotometer shows a negative value at 230 nm and as a result no ratio A260/A230. What causes the negative A230 value?
- My request timer shows negative value in the time remaining until I can request next time and I cannot request. What is going on?
- Can I measure the concentration of ring-shaped DNA on the BioPhotometer?
