How are the data from probing evaluated?
Detection is by enzymatic development of a color, chemiluminescence or fluorescence using any of a variety of methods similar to those used in developing western blots. In addition, radiolabeled probes may be used for example to directly determine sites of phosphorylation using a kinase and ATP32. Arrays may be scanned using a flatbed scanner or by exposure to Xray film. A typical screen of peptides would involve probing for example with a mouse antibody whose epitopes one wishes to determine followed by a labeled second antibody such as HRP conjugated rabbit anti mouse antibody.
