Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

How are the pKD mammalian siRNA expression plasmids made and tested?

expression mammalian PKD plasmids siRNA tested
0
Posted

How are the pKD mammalian siRNA expression plasmids made and tested?

0 CommentsAdd a Comment

1 Answer

0

The DNA oligos are annealed such that they get cloned into the pKD backbone using the SmaI and EcoRI sites. Every pKD plasmid is then tested in tissue culture and its KD ability assessed. We test either by Q-RT-PCR and/or by western blotting.

0 CommentsAdd a Comment

Related Questions

What is your question?

*Sadly, we had to bring back ads too. Hopefully more targeted.