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How can I convert an OD 600 value to cell number/ml?

April 26, 2017CELL convert ML od value

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How can I convert an OD 600 value to cell number/ml?

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Conversion is possible through calibration graphs or counting. With measurements of Escherichia coli, for example, it is possible to estimate roughly that an OD 600 between 0.5 and 1.0 corresponds approximately to a cell number between 1 x 108 and 1 x 109. Additional literature is listed on www.biophotometer.info. Just click on “More information”.