How can I destain the reverse stained gels?
Option 1: Destaining by SDS electrophoresis buffer (regular Tris/Glycine buffer with SDS) for 10 minutes. This destaining method is used where restaining by VisPro 5 minutes Protein Stain or membrane transferring is required. Option 2: Destaining by 10% acetic acid for 5-30 minutes. This destaining method is used where fixing of the protein is required, such as preparing the samples for mass spectrometry (MS) or restaining by other gel staining methods, such as silver stain, CBB stain or Sypro Ruby stain.
