How can I know what sequence is responsible for an RNAi effect?
In order to analyze the effects of a specific siRNA sequence on gene activity, the introduced siRNA sequence must be known. This requires the design, introduction, and measurement of gene blocking following the addition of synthetic siRNA oligonucleotides or of a short hairpin RNA (shRNA) sequence in a vector. When diced siRNA (d-siRNA) are introduced into the cell, they are particularly effective at initiating an RNAi effect because generally there will be several effective siRNA sequences that are part of the pool. However, there currently there is no way to identify the specific sequence(s) in a pool that are responsible for the effect. I only need to knock down gene expression for <7 days First try using Stealth™ RNAi to transfect your cells with Lipofectamine™ RNAiMAX Transfection Reagent I cannot get knockdown in my cells with Stealth™ RNAi and Lipofectamine™ RNAiMAX Your cells may not be transfectable with a lipid. Try using miR RNAi vectors with Adenoviral or Lentiviral deliver
