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How can I monitor the transfection efficiency?

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How can I monitor the transfection efficiency?

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In OriGene we monitor tGFP signal for transfection. PCMV6-mir vector contains an IRES-tGFP reporter gene, which is driven by SV40 promoter and is down-stream of neomycin gene. Independent tGFP and pre-mir expression from different promoters is designed to minimize the interference between the two expression cassettes and therefore tGFP can truly reflect the transfection efficiency.

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