How can I monitor the transfection efficiency?
In OriGene we monitor tGFP signal for transfection. PCMV6-mir vector contains an IRES-tGFP reporter gene, which is driven by SV40 promoter and is down-stream of neomycin gene. Independent tGFP and pre-mir expression from different promoters is designed to minimize the interference between the two expression cassettes and therefore tGFP can truly reflect the transfection efficiency.