How can I tell if the biotinylated oligonucleotide I have made really does contain biotin?
RESPONSE:A colorimetric assay for biotin can be quite effective. The color results from the reaction of biotin with p-dimethylaminocinnamaldehyde in the presence of sulfuric acid. 1. Spot 0.2 A260 units of biotinylated oligonucleotide on a silica gel TLC plate. 2. Dry the plate.] 3. Spray with a solution of 2% p-dimethylaminocinnamaldehyde (Sigma), 2% conc. sulfuric acid in ethanol. 4. Heat the plate and the presence of biotin will be indicated by the formation of a pink spot. Since the intensity of the biotin spot is quite low, it is prudent to compare with an unlabelled oligonucleotide similarly treated. FAQs QUESTION: What is the pressure ratings on our bottles for solvents? RESPONSE:In general, any bottle designed for transportation by needs to be tested to 250kPa (40psi). All of our solvent bottles have been tested to 40psi. Recommended pressures for use should be 5-10psi. As with any glass bottle, a chip may weaken the glass and cause it to burst at lower pressures so precautions
