How can the recovery of hydrophobic peptides be improved?
Insufficient disruption of the binding between the OMIX sorbent and the peptide during the elution step is the primary cause for lower recovery of hydrophobic peptides. Increasing the acetonitrile concentration in the elution solvent to 75-90% will improve the release of these hydrophobic peptides. If elution is being performed with 0.5-2 µL of elution solvent, do not re-aspirate the elution solvent to prevent the hydrophobic peptides from re-binding onto the membrane.
