How clear or clean should my sample be before I begin to use a PhyTip column?
PhyTip columns have been used with a variety of different starting materials, e.g. periplasmic extracts, lysates etc. In all cases we recommend that the solution to be purified is clear of any particulate substances. In order to guarantee this, make sure that the sample to be purified has been spun in a centrifuge so that only the clear supernatant is used. Care should be taken not to disturb the plug of spin down residue while sample processing. After spinning, it is better to transfer the sample to a fresh vial before processing.
