How do I determine the biologically meaningful T(X)?
T(X) is a statistic which provides an indication of the probability with which two distributions are different and also provides a metric by which multiple distributions can be ranked. The higher the value of T(X), the less like the control sample. The number represents how many standard deviations away from the control the test sample is, so a value greater than 4 would represent 99% confidence in a difference. However, the minimum value of T(X) that has biological significance depends on the nature of the data being analyzed and therefore needs to be determined empirically. In order to determine a biologically meaningful T(X), you need to collect samples that you expect to be the same (e.g., the same sample collected twice, the same cell preparation divided and stained in duplicate, or cell preparations from animals of the same strain). Each of these sample pairs measures the variation that can occur between experimental samples. By comparing these pairs, you can determine a biologic
