How do I determine the final yield (amount) of the oligonucleotide?
The computer analysis sheet, provided for each oligonucleotide, gives two conversion factors. The first is nanomoles per OD unit. The second is micrograms per OD unit. (An OD unit is defined as the optical density measured at 260 nanometers.) To find final yield in nanomoles: multiply the “Total A260” (given on the analysis sheet) by the nanomoles per OD conversion factor. The result is total nanomoles yield. To find final yield in micrograms: multiply the “Total A260” (given on the analysis sheet) by the micrograms per OD conversion factor. The result is total micrograms yield.
