Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

How do I generally conduct a T7-based Linear Amplification of DNA (TLDA)?

April 26, 2017amplification conduct dna linear

0

Posted

How do I generally conduct a T7-based Linear Amplification of DNA (TLDA)?

1 Answer

0

Posted

• Start with either ChIP or MeDIP samples. You need around 10ng of DNA per amplification reaction. • If you do not have enough DNA pool your IPs to start TLDA. The amplification typically yields 500 ng/µl (8 µl) of DNA, for a total of 4 µg of DNA. Microarrays usually require 2 µg per microarray.