How do I prepare a firefly luciferase standard curve?
To prepare a luciferase standard curve, use QuantiLum Recombinant Luciferase (Cat.# E1701) diluted in a compatible buffer containing 1mg/ml of BSA as a carrier protein. To choose a buffer compatible with the luciferase assay system to be used, please consult the lysis buffer compatibility table. Dilute the QuantiLum Recombinant Luciferase to create a stock solution of 5 10–12 moles/µl, as shown below. The BioMath Calculator can be used to convert the protein concentration of the 61kDa QuantiLum luciferase to a molar concentration. For example, if the protein concentration of the QuantiLum luciferase is 15.5mg/ml, this would represent a molar concentration of 2.5 1010 mol/µl. Dilute this stock 1:50 to a concentration of 5 1012 mol/µl (tube 1), then do tenfold dilutions to 5 1021 mol/µl. Mix well after each dilution.
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