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How do mutants generated during PCR affect the efficacy of the TAP Express process?

April 26, 2017affect Express mutants PCR process tap

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How do mutants generated during PCR affect the efficacy of the TAP Express process?

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Mutant PCR products produced during the TAP Express process have essentially no effect on gene functionality screening. Even using a polymerase with no proofreading ability, the percentage of mutants generated in the entire PCR fragments pool with a functionally significant effect on protein activity will be negligible.